SARS-CoV PUMC01 滴鼻感染野生小鼠模型

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更新时间：2025-03-16 /

关键词:SARS-CoV PUMC01 滴鼻感染野生小鼠模型 /

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模型信息

中文名称:SARS-CoV PUMC01 滴鼻感染野生小鼠模型

英文名称:SARS-CoV PUMC01 intranasally infected wild-type mice model

类型:SARS-CoV感染动物模型

分级:NA

用途:用于SARS-CoV感染研究。

研制单位:北京协和医院@@http://www.pumch.cn/en.html

保存单位:北京协和医院@@http://www.pumch.cn/en.html

研究背景

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制备方法

hACE2 cDNA was cloned as previously described35 and inserted into the pEGFP-N1 plasmid (BD Biosciences) upstream of the SV40 polyA tract, replacing the coding sequence for enhanced green fl uorescence protein. We retrieved the mouse ACE2 promoter (9 kilobases) by digesting bacterial artifi cial chromosome (RP23-75P20) DNA with KpnI and EcoRV and inserted it upstream of the hACE2 coding sequences. After hACE2 was identifi ed in transfected NIH3T3 cells, the plasmid was linearized with KpnI and KasI and purifi ed with phenol. Fragments carrying the mouse ACE2 promoter driving the hACE2 coding sequence were introduced by microinjection into the pronuclei of fertilized ova from ICR mice. The injected zygotes then were transplanted into the oviducts of pseudopregnant mice. Mice in which the transgene integrated were identifi ed from tail biopsies. These mice were bred to produce offspring for analysis.